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To examine the impact of Nlg1 tyrosine phosphorylation on the binding of Nlg1 to full length gephyrin or PSD-95, we performed pull-down experiments using recombinant GST proteins fused to the intracellular domain of Nlg1, either from wild-type Nlg1 (Nlg1-WT), or from Nlg1 carrying Y782A or Y782F mutations (Fig
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