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ABTS •+ may be generated from ABTS in the presence of powerful antioxidant agents

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As used herein, the term“chelated preparation” or“chelated solution” refers to a preparation or solution treated with a chelating agent in an amount and for a time sufficient to diminish the reactivity of a metal ion in the solution by at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or 100%