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By contrast, CDT exploits endogenous chemical conditions without external energy delivery but sacrifices some temporal control and depends strongly on substrate availability
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Another possibility for pH-independent sensing of H 2 O 2 levels inside cells has been reported with roGFP2-based probes that use the yeast glutathione peroxidase Orp1 (also named Gpx3) or a single-cysteine variant of the peroxiredoxin Tsa2 as redox-reactive specificity domains [15, 22]
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