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reliable data could not be obtained at 5-amino-1MQ concentrations above 100 ⁇ M due to inference with the NAMPT assay readout signal (Table 6)

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Biochemical and omics biomarkers in cognition Classical CSF and plasma biomarkers Aβ40 / Aβ42 (important ratio in amyloid pathology) Total tau / p-tau181 / p-tau217 (tauopathic markers) Neurofilament light (NfL) : axonal degeneration marker GFAP : astrocytic activation BDNF and pro-BDNF : neurotrophic activity IL-6, TNFα, IL-1β : neuroinflammation Transcriptomics and epigenomics Hippocampal RNA-seq after chronic administration of Semax or Selank often reveals a neurotrophic activation signature (BDNF, NT3, NGF, NR4A1-3) coupled with anti-inflammatory modulation (NF-κB targets down)