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Use of Growth Hormone, IGF-I, and Insulin for Anabolic Purpose: Pharmacological Basis, Methods of Detection, and Adverse Effects - PMC
doi: 10.4103/1673-5374.241445, PMID: [DOI] [PMC free article] [PubMed] [Google Scholar] Murakami T., Ilieva H., Shiote M., Nagata T., Nagano I., Shoji M
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The soluble fraction was loaded onto a nickel affinity column formed from nickel sepharose beads (GE Biosciences) pre-equilibrated with lysis buffer
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