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After boiling, 450 uL of ice cold 1X Lysis buffer (1X RIPA, supplemented with 1X protease inhibitor, 1X phosphatase inhibitor, 50 mM N-Ethylmalamide) was then added to the sample, and cells were left on ice for 20 min with vortexing every 5 min for 10 s
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doi: 10.1074/jbc.270.20.11977 [DOI] [PubMed] [Google Scholar] 86.Alam J, Igarashi K, Immenschuh S, et al
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