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The library consisted of 6,101 individual expression vectors encoding full-length human plasma membrane proteins, secreted proteins, and cell surface-tethered human secreted proteins, plus 396 human heterodimers
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Freshly prepared AX in PBS was incubated in the absence or presence of purified HSA purified from serum for 16 h at 37°C (final concentrations of HSA and AX in incubation mixture were 3 mg/ml and 2.5 mg/ml, respectively)
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