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To evaluate the neuroprotective effects of compounds, a neuronal culture medium was replaced with a Neurobasal medium without neurotrophic factors supplemented with 100 µM deferoxamine (DFO concentration optimum determined based on dose curve at 10 °C) (Sigma-Aldrich), 100 nM BAM15 (Tocris, Bristol, UK), 1:500 dilution of protease inhibitor (PI) cocktail III (Sigma-Aldrich)
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[DOI] [PMC free article] [PubMed] [Google Scholar] 52.Gaasch JA, Lockman PR, Geldenhuys WJ, Allen DD, Van der Schyf CJ
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