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Dysregulated Kras/YY1/ZNF322A/Shh transcriptional axis enhances neo-angiogenesis to promote lung cancer progression

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The peptides were eluted onto a capillary column (ZORBAX Extend-C18, 80 Å, 4.6 × 250 mm, 5 µM) and separated at a flow rate of 0.5 ml min −1 using the following gradient with buffer A (10 mM aqueous NH 4 HCO 3 ) and buffer B (100% acetonitrile): 100% buffer A 0–2 min, 0–13% buffer B 2–3 min, 13–42% buffer B 3–60 min, 42–100% buffer B 60–61 min, 100% buffer B 61–65 min, 100–0% buffer B 65–66 min, 100% buffer A 66–75 min, 0–13% buffer B 75–78 min, 13–80% buffer B 78–80 min, 80% buffer B 80–85 min, 100% buffer A 86–91 min, 0–13% B from 91–94 min, 13–80% buffer B 94–96 min, 80% buffer B 96–101 min and 80–0% buffer B 101–102 min