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In accordance with the increased Ac-PGC1α levels, cardiac SIRT1 protein levels were decreased in mice treated with HFD supplemented with tesaglitazar (–61%

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After incubation, cells were washed with PBS twice, re-suspended, and immediately submitted to flow cytometric analysis using a BD Accuri C6 Plus flow cytometer (Becton Dickinson, Mountain View, CA, USA)