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Walkersville, MD, United States), which consisted of smooth muscle basal medium, 5% FBS, 5 μg/ml insulin, 2 ng/ml human fibroblast growth factor, and 0.5 ng/ml human epidermal growth factor, for 5–7 days before each experiment

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However, the FDA emphasizes that the preparation of compounded medication should be done in state or federal pharmacies by licensed pharmacists, healthcare providers, and people working under the direct supervision of licensed pharmacists