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The liver tissue was homogenized with a buffer solution having 250 mM sucrose, 50 mM Tris-HCl (pH 7.4), and 1 mM EDTA and stored with 4% paraformaldehyde (PFA) or at –80°C
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They are less accurate than lab analysis, so a positive home result is best confirmed by a lab, and anyone testing because of illness or flooding should rely on a lab from the start
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