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Chromatography was performed on a 2.1 × 150 mm, 1.7 μm, BEH C18 column (Waters) at 45 °C using aqueous HCOOH (0.1% in H 2 O) as solvent A and MeCN containing 0.1% HCOOH as solvent B and the following gradient (flow rate: 0.4 mL/min): For dipeptide analysis in the hydrolysates, chromatography started with 1% solvent B for 2.5 min, which was increased to 95% within 5.5 min, hold for 1.5 min and, then, decreased again to 1% within 0.5 min

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DSIP stands out for its direct effects on sleep architecture and its unique combination of sleep-promoting and stress-modulating properties