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PDF) Glutathione Precursor Supplementation with Immunocal® for Inflammaging: An Open-Label Pilot Study Assessing suPAR Modulation in Middle-Aged Adults

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Reverse transcription of RNA samples to generate cDNA was performed with the reverse transcription reagent Evo M-MLV RT Master Mix (Agbio, Hunan, China), which was conducted as follows: 37°C for 15 min, 85°C for 5 s and hold at 4°C