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The remainder of the donor and crRNA reaction was combined, EtOH purified, resuspended in 50 µl ultrapure water, heat-sterilized at 95°C for 5 min, and then used for transfection

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Furthermore, FRDA patient heart, liver, and spleen sections analyzed for iron content using Perl’s stain demonstrate extra-nuclear iron deposition, and in a chain-like organization, indicating mitochondrial localization ( Similarly, a FRDA mouse model of cardiac and skeletal muscle FXN loss demonstrates in cardiac tissue: increased TfR, decreased cytoplasmic ferritin heavy and light chain subunits, increased mitochondrial iron importer mitoferrin-2 (mfrn-2), and decreased expression of the iron exporter ferroportin (FPN) (Huang et al., 2009)