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The blocking solution was removed and 150 μL primary antibody (71–1,500, Rabbit Occludin Polyclonal Antibody, ThermoFisher Scientific) diluted in blocking solution (8.33 μg/mL) was added
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The second dominant phylum Bacteroidetes is mainly represented by the genera Bacteroides and Prevotella ( The microbial community provides numerous benefits for the host contributing to metabolism of nutrients, drugs, xenobiotics, maintenance of gut barrier function and intestinal immune homeostasis (Table 1 summarizes information regarding the experimental conditions and the identified microbiota-produced GRP metabolites
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