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During this period, lactogenesis, the regression of the uterus and the accompanying changes in hormone balance and metabolism, present the dairy cow with unique adaptive challenges that are particularly dramatic for the transition dairy cows ( High levels of reactive oxygen species (ROS) in periparturient dairy cows could expose them to increased oxidative stress, which contribute to dysfunctional immune responses and increased risk of health disorders in cows and their calves ( 12 and E) and Minerals (zinc, copper, selenium, and iron) are essential for dairy cows’ health and production, as they work as cellular antioxidants and immune system modulators ( One of the major drawbacks of oral intake of micronutrients in cattle is reduced absorption due to interactions with other nutrients at the rumen level ( 2 Materials and methods 2.1 Selection of cows, health and feeding management The present study was conducted on 24 peripartum healthy Karan Fries (Holstein Friesian X Tharparkar) cows at the Livestock Research Centre of National Dairy Research Institute, Karnal, India, situated 245 m above mean sea level in the bed of Indo-Gangetic alluvial plain, latitude 29° 43’ N and longitude 77° 2’ E