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In the later stages of nitrogen deficiency stress (S3 stage, 48 DAT), the leaves transition from a storage reservoir of nutrients to a source of nutrient transport, with enhanced decomposition and senescence, and are discarded to decompose and produce nitrogen sources for other organs ( Figure 3 )

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Ultimately, FeS@SRB with highly active and highly FeS-loaded amounts were prepared at an initial Fe 2+ concentration of 200 mg L −1 for 48 h at 37 °C according to the experimental results and were used for subsequent studies