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Subsequently, according to the method described in the previous study ( 2.7 Immunofluorescence (IF) After transcardial perfusion in mice, brain tissues were harvested and pretreated with gradient dehydration in 20% and 30% sucrose solutions at 4°C (to reduce ice crystal damage), then embedded in optimal cutting temperature compound and sectioned for 20 μm thickness using cryostat

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Our results support that axon injury and ferroptosis signaling triggers PSR-1 condensation on the axonal membrane to facilitate axonal fusion (Fig