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Sensitive quantitative techniques for measuring glutathione in vitro are available by both HPLC and spectrophotometric enzymatic assay, while other methods have been developed to provide information on glutathione in situ (Figure 2)
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The NaCl aerosol was generated by atomization (and subsequent drying) of a solution containing 1.0 g NaCl (purity ≥ 99.0%, Sigma-Aldrich, USA) in 500 ml nanopure water, to give particles in the size range 40–150 nm
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