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For hexanucleosomes reconstituted without H1, the buffer was adjusted to 2.5 and 3.0 mM MgCl 2
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Size:4 OZ.
Cells were collected by centrifugation and transferred to MMg solution (400 mm mannitol, 10 mm MgCl 2 , 4 mm MES pH 5.7) for transfection
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