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Chelating agents in a buffer will strip copper from the complex, converting it back toward free peptide, so buffer composition is not a neutral choice for this material
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For analysis, 50 ยตl of 40 mM HEPES pH=7.4, 1 mM EDTA and 6 M Guanidine HCl buffer was added to the tubes with worms, frozen animals grinded with a disposable pestle and lysed by two freeze thaw cycles
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