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Understanding Epstein-Barr Virus (EBV)

The extracted pigments were saponified with 25 ml of 10% (v/w) methanolic potassium hydroxide for 2 h at room temperature, then the carotenoids and tocopherols were extracted with dichloromethane

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Briefly, increasing volumes of solutions of the studied compounds or the garlic extract were added to wells of a 96-well microplate pre-filled with 200 μL of the working solution composed of 0.3 M acetate buffer, pH 3.6 (10 volumes), 10 mM TPTZ in 40 mM HCl (1 volume), and 20 mM FeCl 3 (1 volume), prepared immediately before use