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BPC 157 Therapy and the Permanent Occlusion of the Superior Sagittal Sinus in Rat: Vascular Recruitment - PMC
The beads were washed with 15 mL of wash buffer (50 mM Tris pH 7.4, 300 mM NaCl, 0.5 mM DTT, 20 mM imidazole) and then with 5 mL elution buffer (50 mM Tris pH 7.4, 300 mM NaCl, 0.5 mM DTT, 500 mM imidazole)
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