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For all experiments, cultured THP-1 cells were treated with CaOx, Cystine or CaP crystals (50, 100, 200, 500, 1000 µg/ml) or NaOx (0.1, 0.5, 1, 1.5, and 2 mM) and incubated at 37 °C in 5% CO 2 for 24 h